Propeptide-luciferase fusion proteins and methods of use thereof

US10370697B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• The General Hospital Corporation · US
• INSTITUTO CARLOS SLIM DE LA SALUD, A.C. · MX

Inventors

• Sean Burns · Hingham, US
• David Altshuler · Brookline, US
• Amedeo Vetere · Cambridge, US

Key dates

• Filing date: May 19, 2017
• Grant date: Aug 6, 2019
• Expiry date: May 19, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/60
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides nucleic acid constructs that encode fusion peptides comprising a bioluminescent protein and a precursor of a secreted peptide or protein expressed at the cell surface and high throughput screening assays using same.