Optimized method for antibody capturing by mixed mode chromatography

US10377794B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Roberto Falkenstein · Munich, DE
• Thorsten Lemm · Kochel am See, DE
• Markus Strasser · Penzberg, DE
• Hidenari Yamada · Tokyo, JP

Key dates

• Filing date: Jul 21, 2016
• Grant date: Aug 13, 2019
• Expiry date: May 28, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K16/2863
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Herein is reported a method for the purification of an antibody directly captured from clarified cell culture supernatants using Streamline CST and/or Capto MMC, wherein especially product related (aggregates and fragments) and process related impurities (host cell protein, media components) could efficiently be removed, resulting in a preparation with a purity comparable to classical protein A affinity chromatography.