Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

US10385345B2 · kind B2 · utility

Assignees

• Lallemand Hungary Liquidity Management LLC · HU
• Universiteit Stellenbosch · ZA

Inventors

• Elena Brevnova · Belmont, US
• John McBride · Lyme, US
• Erin Wiswall · Danbury, US
• Kevin S. Wenger · Rolesville, US
• Nicky Caiazza · Rancho Santa Fe, US
• Heidi Hau · Lebanon, US
• Aaron Argyros · Lebanon, US
• Frank Agbogbo · Lebanon, US
• Charles F. Rice · Plainfield, US
• Trisha Barrett · Bradford, US
• John S. Bardsley · Newport, US
• Abigail Foster · South Strafford, US
• Anne K. Warner · Lebanon, US
• Mark Mellon · Lebanon, US
• Ryan Skinner · White River Junction, US
• Indraneel Shikhare · Lebanon, US
• Riaan Den Haan · Durbanville, ZA
• Chhayal V. Gandhi · Lebanon, US
• Alan Belcher · Nashua, US
• Vineet Rajgarhia · Dublin, US
• Allan Froehlich · Hartland, US
• Kristen M. Deleault · Canaan, US
• Emily Stonehouse · Lebanon, US
• Shital A. Tripathi · Berkeley, US
• Jennifer Gosselin · Lebanon, US
• Yin-Ying Chiu · Lebanon, US
• Haowen Xu · Lebanon, US

Key dates

• Filing date: May 2, 2017
• Grant date: Aug 20, 2019
• Expiry date: May 2, 2037

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to…