Resolution enhancement for light sheet microscopy systems and methods

US10401604B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Hari Shroff · Rockville, US
• Yicong Wu · North Potomac, US
• Sara Abrahamsson · Rockville, US

Key dates

• Filing date: Sep 24, 2015
• Grant date: Sep 3, 2019
• Expiry date: Dec 24, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2201/06113
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Embodiments of a resolution enhancement technique for a light sheet microscopy system having a three objective lens arrangement in which one objective lens illuminates a sample and the second and third objective lenses collect the fluorescence emissions emitted by the sample are disclosed. The second objective lens focuses a first portion of the fluorescence emissions for detection by a second detection component, while the third objective lens focuses a second portion of the fluorescence emissions through a diffractive or refractive optic component for detection by a first detector component. A processor combines the images resulting from the first and second portions of the fluorescence emissions for generating composite images with increased axial and lateral resolution.