Purifying insulin using cation exchange and reverse phase chromatography in the presence of an organic modifier and elevated temperature
US10407483B2 · kind B2 · utility
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Key dates
| Filing date | Mar 11, 2015 |
| Grant date | Sep 10, 2019 |
| Priority date | — |
| Expiry date | Jun 24, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is described for purifying insulin and insulin analogs that comprises high-pressure liquid chromatography with an acidic cation exchange medium performed in the presence of a water miscible organic modifier and at an elevated temperature followed by reverse phase chromatography performed in the presence of a water miscible organic modifier and at an elevated temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.