Patent · US Active

Method for improving antibody stability

US10418126B2 · kind B2 · utility

0Cited by

2References

6Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Hubert Kettenberger · Munich, DE
Stefan Klostermann · Schwanau, DE
Florian LIPSMEIER · Basel, CH
Apollon Papadimitriou · Bichl, DE
Jasmin SYDOW-ANDERSEN · Copenhagen, DK

Key dates

Filing date	Mar 4, 2016
Grant date	Sep 17, 2019
Priority date	—
Expiry date	Mar 4, 2036

Classification

Technology area (CPC G)Physics
CPC primaryG01N2500/00
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Herein is reported a method for selecting or deselecting an antibody comprising a) determining for each Asp and Asn residue in an antibody Fv region the conformational flexibility of the Cα-atom using a homology model ensemble, b) determining for each Asp and Asn residue in an antibody Fv region the size of the amino acid residue C-terminal to the Asp or Asn residue, and c) selecting an antibody in which the Cα-atom is conformationally inflexible and/or the Asp or Asn has a big C-terminal amino acid residue, or deselecting an antibody in which the Cα-atom has a moderate to high conformational flexibility and/or the Asp or Asn has a small C-terminal amino acid residue.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.