Pseudomonas exotoxin A with less immunogenic T cell and/or B cell epitopes

US10428119B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Ira H. Pastan · Potomac, US
• Ronit Mazor · Rockville, US
• Masanori Onda · Germantown, US
• Aaron Vassall · Shelton, US
• Richard Beers · Rockville, US
• Jaime Eberle · Delmar, US
• Wenhai Liu · Plantsville, US

Key dates

• Filing date: Sep 1, 2017
• Grant date: Oct 1, 2019
• Expiry date: Sep 1, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/55
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention provides a Pseudomonas exotoxin A (PE) comprising an amino acid sequence having a substitution of one or more B-cell and/or T-cell epitopes. The invention further provides related chimeric molecules, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions. Methods of treating or preventing cancer in a mammal, methods of inhibiting the growth of a target cell, methods of producing the PE, and methods of producing the chimeric molecule are further provided by the invention.