Patent · US Active

Method of culturing segmented filamentous bacteria in vitro

US10435665B2 · kind B2 · utility

3Cited by
0References
45Claims
0Family size

Assignees

Inventors

Key dates

Filing dateDec 23, 2015
Grant dateOct 8, 2019
Priority date
Expiry dateDec 23, 2035

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY02A50/30
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention relates to an in vitro method of culturing a segmented filamentous bacterium strain, comprising co-culturing said segmented filamentous bacterium strain with a eukaryotic host cell, wherein the culture is performed at an O2 level inferior to 5% in a rich tissue culture liquid medium containing bacterial medium components including iron. The present invention also relates to methods for genetically modifying a segmented filamentous bacterium strain comprising a step a culturing the strain in vitro.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.