Cyclopentane-peptide nucleic acids for qualitative and quantitative detection of nucleic acids

US10457978B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Daniel H. Appella · Rockville, US
• Christopher Michael Micklitsch · Philadelphia, US
• Bereket Yemane · Bound Brook, US
• Chao Zhao · Thuwal, SA

Key dates

• Filing date: Aug 16, 2013
• Grant date: Oct 29, 2019
• Expiry date: Nov 27, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/131
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns methods for detecting a nucleic acid of interest in a solution comprising (a) contacting a solution suspected of containing the nucleic acid of interest with a PNA capture probe and a PNA reporter probe; wherein (i) the PNA capture probe comprises at least two trans-cyclopentanes; (ii) the PNA reporter probe comprises at least six biotin groups; (iii) the PNA capture probe bound to a surface; and (iv) the PNA capture probe and the PNA reporter probe each comprise a nucleobase sequence that is complementary to different non-overlapping portions of the nucleic acid of interest; (b) detecting the presence of the PNA capture probe and the PNA reporter probe bound to the surface; wherein the nucleic acid of interest is detected when 1-1000 molecules of the nucleic acid of interest are present in the solution being tested.