Cantilever sensors for molecule detection
US10458980B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 23, 2016 |
| Grant date | Oct 29, 2019 |
| Priority date | — |
| Expiry date | Jan 2, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/51
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of preparing a cantilever sensor for measuring biochemical interactions and their associated stress wherein a cantilever having two sides is coated on one side with at least a gold layer and both sides of the cantilever are functionalized with a self-assembled monolayer (SAM) of a probe molecule by incubating the cantilever in a solution having a concentration of the probe molecule of between 1 to 1000 μM. The unpassivated cantilever sensor comprising a layer coated on one side with a coating comprising gold and being unpassivated on the opposite side, wherein both surfaces comprises a self-assembled monolayer of a probe molecule in which the surface area occupied per probe molecule varies in the range 0.4-1.5 nm2, enabling the stress at the gold top surface that is not cancelled out by a counter stress from the bottom surface so that accurate quantitation of a target molecule is achieved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.