Three-step acid dissociation enzyme linked immunosorbent (TADELIS) assay

US10458981B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Gregor Jordan · Penzberg, DE
• Roland Staack · Penzberg, DE

Key dates

• Filing date: Jun 15, 2018
• Grant date: Oct 29, 2019
• Expiry date: Jun 15, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/94
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Herein is reported a method for determining in a sample the (total, i.e. binding competent) amount of a ligand of a ligand-binding protein (therapeutic) comprising the following steps in the following order: subjecting the sample to an acid treatment, forming in solution a non-covalent complex comprising i) an anti-ligand antibody, ii) the ligand, and iii) labelled ligand-binding protein, by adding anti-ligand antibody and labelled ligand-binding protein to the sample, and determining the amount of the complex and thereby determining the amount of the ligand of the ligand-binding protein.