Nucleic acid constructs and methods for labeling and detecting nucleosomal DNA modifications

US10479986B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• The General Hospital Corporation · US

Inventors

• Charles B. Epstein · Brookline, US
• Peter van Galen · Boston, US
• Bradley E. Bernstein · Cambridge, US

Key dates

• Filing date: May 26, 2017
• Grant date: Nov 19, 2019
• Expiry date: May 26, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Embodiments disclosed herein provide methods for identifying cell-type-specific nucleosomal DNA modifications. The methods leverage nucleosomal DNA barcoding and pool-and-split multiplexing to provide high-throughout, quantitative profiling of nucleosomal DNA states. The methods enable the profiling of multiple nucleosomal DNA marks across different cell types and/or conditions thereby linking quantitative changes in chromatin landscapes to different genotypes and chemical and physical perturbations.