Crispr enzyme mutations reducing off-target effects

US10494621B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US

Inventors

• Feng Zhang · Cambridge, US
• Linyi Gao · Longlane, GB
• Bernd Zetsche · Cambridge, US
• Ian Slaymaker · Cambridge, US

Key dates

• Filing date: Oct 11, 2018
• Grant date: Dec 3, 2019
• Expiry date: Oct 11, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed and claimed are mutation(s) or modification(s) of the CRISPR enzyme, for example a Cas enzyme such as a Cas9, which obtain an improvement, for instance a reduction, as to off-target effects of a CRISPR-Cas or CRISPR-enzyme or CRISPR-Cas9 system or complex containing or including such a mutated or modified Cas or CRISPR enzyme or Cas9. Methods for making and using and uses of such mutated or modified Cas or CRISPR enzyme or Cas9 and systems or complexes containing the same and products from such methods and uses are also disclosed and claimed.