Disulfide-linked multivalent MHC class I comprising multi-function proteins

US10501521B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Guy Georges · Habach, DE
• Sabine Imhof-Jung · Planegg, DE
• Hendrik Knoetgen · Penzberg, DE
• Martina Schmittnaegel · Tutzing, DE

Key dates

• Filing date: Dec 18, 2013
• Grant date: Dec 10, 2019
• Expiry date: Dec 18, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/16133
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Herein is reported a disulfide-linked multivalent multi-function protein, characterized in that it comprises two or more antigen presenting domains, exactly one antibody Fc-region, and at least one antigen binding site, wherein the antigen presenting domain comprises in N- to C-terminal direction either (i) a β2-microglobulin, and (ii) the extracellular domains α1, α2, and α3 of a class I MHC molecule with a relative frequency of less than 1%, or (i) a T-cell response eliciting peptide, (ii) a β2-microglobulin, and (iii) the extracellular domains α1, α2, and α3 of a class I MHC molecule with a relative frequency of 1% or more, wherein the antigen binding site binds to a cancer cell surface antigen or a virus-infected cell surface antigen and wherein the antigen presenting domain has at least two non-naturally occurring cysteine residues which form an intrachain/interdomain disulfide bond.