Using serial dilutions of reference samples to construct a reference table for sigmoidal fitting in real-time PCR copy number analysis
US10510436B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 5, 2016 |
| Grant date | Dec 17, 2019 |
| Priority date | — |
| Expiry date | Apr 17, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B25/20
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
The present invention discloses a method of real-time quantification of a target nucleic acid in a sample by constructing a reference table of copy number vs. designated parameter from reference samples which sharing the same nucleic acid sequences with the target nucleic acid. The method includes (a) constructing a reference table of copy number vs. designated parameter from reference samples; (b) amplifying the target nucleic acid; (c) monitoring and detecting the amplification of the target nucleic acid in real-time; (d) analyzing the detected signals to get the designated parameter of the target nucleic acid; and (e) looking up and interpolating to the reference table to get the copy number of the target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.