Sample preparation, processing and analysis systems

US10525467B2 · kind B2 · utility

Assignee

• INTEGENX INC. · US

Inventors

• William D. Nielsen · San Jose, US
• Richard J. Belcinski · San Jose, US
• Gregory Bogdan · San Jose, US
• David Eberhart · Santa Clara, US
• Omar El-Sissi · Fremont, US
• Stevan B. Jovanovich · Livermore, US
• Michael Recknor · Oakland, US
• Ezra Van Gelder · Palo Alto, US
• David W. Wyrick · Livermore, US

Key dates

• Filing date: Nov 3, 2016
• Grant date: Jan 7, 2020
• Expiry date: Jul 13, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2035/0091
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.