Method for the biotechnological production of flavone glycoside dihydrochalcones

US10538797B2 · kind B2 · utility

Assignee

• SYMRISE AG · DE

Inventors

• Maren Thomsen · Greifswald, DE
• Jakob Ley · Holzminden, DE
• Egon Gross · Holzminden, DE
• Winfried Hinrichs · Greifswald, DE
• Uwe Bornscheuer · Greifswald, DE

Key dates

• Filing date: Jun 27, 2015
• Grant date: Jan 21, 2020
• Expiry date: Jul 13, 2035

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A23V2002/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A′) according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A′) according to SEQ ID NO:4 has been cut out.