Patent · US Active

Bacterial endotoxin test for the determination of endotoxins

US10585097B2 · kind B2 · utility

2Cited by

4References

52Claims

0Family size

Assignees

Forschungszentrum Borstel Leibniz Lungenzentrum · DE
Hoffmann-La Roche Inc. · US

Inventors

Christian Alexander · Kellinghusen, DE
Sven Deutschmann · Weilheim, DE
Pierre Lang · Ecublens, CH
Friedrich Von Wintzingerode · Penzberg, DE
Ulrich Zaehringer · Ahrensburg, DE

Key dates

Filing date	Jul 27, 2016
Grant date	Mar 10, 2020
Priority date	—
Expiry date	Jul 27, 2036

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY02A50/30
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Herein is reported a method for determining bacterial endotoxin at low concentrations in a sample of an antibody (that has been produced using bacterial cells) comprising the following steps in the following order: i) adding magnesium ions to the sample, ii) diluting the sample, iii) dialyzing the sample having a pH-value of 5.7-8.0 against an endotoxin-free aqueous solution, and iv) determining bacterial endotoxin in the sample using a bacterial endotoxin test, particularly the limulus amoebocyte lysate assay.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.