Method for generating a reflection-reduced contrast image and corresponding device

US10620417B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Lars Stoppe · Jena, DE
• Thomas MILDE · Nausnitz, DE
• Johannes Winterot · Jena, DE
• Christoph Husemann · Jena, DE

Key dates

• Filing date: Apr 27, 2016
• Grant date: Apr 14, 2020
• Expiry date: Jan 1, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/20221
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

The invention relates to a reflection-reduced contrast imaging method and to a device for generating a reflection-reduced contrast image, preferably from microscopic images, in particular in order to read height progression information of a condition of an object. The reflection-reduced contrast imaging method comprises: illuminating an object by means of an illumination sequence, creating an illumination image of the object for each illumination of the object of the illumination sequence, determining a reflection-corrected illumination image for each illumination image, superposing each pair of reflection-corrected illumination images that are adjacent with respect to the first axis in order to form a first whole axis image of the first axis, superposing each pair of reflection-corrected illumination images that are adjacent with respect to the second axis in order to form a second whole axis image of the second axis, creating a first color progression image on the basis of the first whole axis image, creating a second color progression image on the basis of the second whole axis image, transforming the first color progression image and the second color progression image into a co…