Arrangement for light sheet microscopy

US10620419B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Jörg Siebenmorgen · Jena, DE
• Thomas Kalkbrenner · Jena, DE
• Helmut Lippert · Jena, DE

Key dates

• Filing date: Jul 8, 2014
• Grant date: Apr 14, 2020
• Expiry date: Jul 8, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B27/0068
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An arrangement for light sheet microscopy including: a sample vessel, for receiving a medium containing sample, having a covering and being oriented with respect to a planar reference surface; illumination optics with an illumination objective for illuminating the sample with a light sheet; and detection optics with a detection objective. The optical axis of the illumination objective and the light sheet lies in a plane that forms a nonzero illumination angle with the normal of the reference surface. The optical axis of the detection objective forms a nonzero detection angle with the normal of the reference surface. A bulge is formed at the covering for receiving the sample. The bulge has inner and outer interfaces. The optical axes of the illumination objective and detection objective form a minimal angle with the normals of the interfaces at least in the region where the optical axes pass through the interfaces.