System for generating a synthetic 2D image with an enhanced depth of field of a biological sample
US10623627B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 22, 2017 |
| Grant date | Apr 14, 2020 |
| Priority date | — |
| Expiry date | Feb 22, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/244
- WIPO fieldAudio-visual technology
- WIPO sectorElectrical engineering
Abstract
The present invention relates to a system for generating a synthetic 2D image with an enhanced depth of field of a biological sample. It is described to acquire (110) with a microscope-scanner (20) first image data at a first lateral position of the biological sample and second image data at a second lateral position of the biological sample. The microscope-scanner is used to acquire (120) third image data at the first lateral position and fourth image data at the second lateral position, wherein the third image data is acquired at a depth that is different than that for the first image data and the fourth image data is acquired at a depth that is different than that for the second image data. First working image data is generated (130) for the first lateral position, the generation comprising processing the first image data and the third image data by a focus stacking algorithm. Second working image data is generated (140) for the second lateral position, the generation comprising processing the second image data and the fourth image data by the focus stacking algorithm. The first working image data and the second working image data are combined (150), during acquisition of image …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.