Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
US10655173B2 · kind B2 · utility
3Cited by
10References
12Claims
0Family size
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Key dates
| Filing date | Oct 17, 2014 |
| Grant date | May 19, 2020 |
| Priority date | — |
| Expiry date | Oct 17, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6846
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to molecular microscopy or volumetric imaging by proximal unique molecular identifiers (“UID”) reaction (“VIPUR”) microscopy methods to record the cellular co-localization and/or spatial distributions of arbitrary nucleic acid sequences, or other biomolecules tagged with nucleic sequences. The method involves one or both of two DNA sequence-components such as an α-UID, which may identify the targeted sequences-of-interest themselves and/or spatial beacons relative to which their distances are measured, and a- β-UID, which labels α-UID association events.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.