Human liver scaffolds
US10688221B2 · kind B2 · utility
2Cited by
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12Claims
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Assignee
Inventors
Key dates
| Filing date | Jun 2, 2015 |
| Grant date | Jun 23, 2020 |
| Priority date | — |
| Expiry date | Apr 4, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/085
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to methods for decellularising human liver tissue to produce human hepatic extracellular matrix (ECM) scaffolds, for example for use in therapy or disease modelling. The methods involve mechanically damaging cells in the tissue, for example by freeze thaw, and then subjecting the liver tissue to multiple cycles of osmotic stress, detergent treatment and protease and/or DNAase treatment to produce a decellularised human ECM scaffold.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.