Unbiased identification of double-strand breaks and genomic rearrangement by genome-wide insert capture sequencing

US10689691B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US

Inventors

• Feng Zhang · Cambridge, US
• Winston X. Yan · Brookline, US
• David A. Scott · Cambridge, US
• Aaron Smargon · Cambridge, US
• Mohammed Reza Mirzazadeh · Stockholm, SE
• Nicola Crosetto · Stockholm, SE

Key dates

• Filing date: Jun 19, 2017
• Grant date: Jun 23, 2020
• Expiry date: Jun 19, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides for systems, methods, compositions, and kits for the complete characterization of targeted nuclease specificity which necessitates techniques that can assess the full possibility space of off-target activity and genomic stability following genome editing. Also provided are the materials and techniques which enable the comprehensive genomic stability accompanying a range of cellular perturbations, including genome editing (ZFN, TALEN, CRISPR, and future technologies) and disease modeling among other applications.