Optimized CRISPR-Cas double nickase systems, methods and compositions for sequence manipulation
US10711285B2 · kind B2 · utility
3Cited by
14References
36Claims
0Family size
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Key dates
| Filing date | Dec 17, 2015 |
| Grant date | Jul 14, 2020 |
| Priority date | — |
| Expiry date | Mar 25, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/80
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.