Ionic strength-mediated pH gradient ion exchange chromatography
US10712322B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 29, 2017 |
| Grant date | Jul 14, 2020 |
| Priority date | — |
| Expiry date | Jan 10, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6803
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides methods for analyzing compositions of polypeptides such as antibodies by ionic strength-mediated pH gradient ion exchange chromatography. In some aspects, the methods use a combination of pH gradients and ionic strength gradients to separate the polypeptide from charge variants of the polypeptide. In some aspects, the methods use a stable ionic strength to optimize the pH gradient separation window to separate the polypeptide from charge variants. Such methods are useful for analyzing polypeptide, e.g. antibodies, with a pI greater than 9 or a pI less than 7. In some aspects, the invention provides a multiproduct method for the analysis of polypeptides of varying pI's.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.