Spatial molecular analysis of tissue
US10724089B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 6, 2017 |
| Grant date | Jul 28, 2020 |
| Priority date | — |
| Expiry date | Jun 12, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Various methods and devices for spatial molecular analysis from tissue is provided. For example, a method of spatially mapping a tissue sample is provided with a microarray having a plurality of wells, wherein adjacent wells are separated by a shearing surface; overlaying said microarray with a tissue sample; applying a deformable substrate to an upper surface of said tissue sample; applying a force to the deformable substrate, thereby forcing underlying tissue sample into the plurality of wells; shearing the tissue sample along the shearing surface into a plurality of tissue sample islands, with each unique tissue sample island positioned in a unique well; and imaging or quantifying said plurality of tissue sample islands, thereby generating a spatial map of said tissue sample. The imaging and/or quantifying may use a nucleic acid amplification technique.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.