Functional genomics using CRISPR-Cas systems, compositions, methods, screens and applications thereof

US10781444B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US

Inventors

• Feng Zhang · Cambridge, US
• Ophir Shalem · Albany, US
• Neville Espi Sanjana · New York, US
• John Doench · Cambridge, US
• David E. Root · Santa Rosa, US
• Ella Nicole Biewener Hartenian · Brookline, US

Key dates

• Filing date: Dec 17, 2015
• Grant date: Sep 22, 2020
• Expiry date: Dec 17, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention generally relates to libraries, compositions, methods, applications, kits and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.