Patent · US Active

Multifocal scanning fluorescence microscope

US10802256B2 · kind B2 · utility

2Cited by

4References

18Claims

0Family size

Assignees

Carl Zeiss Microscopy GmbH · DE
Carl Zeiss AG · DE

Inventors

Tiemo Anhut · Jena, DE
Daniel Schwedt · Weimar, DE
Matthias Wald · Jena, DE

Key dates

Filing date	Feb 9, 2015
Grant date	Oct 13, 2020
Priority date	—
Expiry date	Dec 24, 2035

Classification

Technology area (CPC G)Physics
CPC primaryG02B27/4227
WIPO fieldOptics
WIPO sectorInstruments

Abstract

Scanning fluorescence microscopes with an observation beam path from a measurement volume to an image plane. A beam combiner is provided for coupling an illumination system and a diaphragm arranged in the image plane for slow composition of the image because of the sequential scanning and subject the sample to loading as a result of inefficient use of the excitation light. The microscope simultaneously detects fluorescence from different focal planes in each case quasi-confocally. The observation beam path between the beam combiner and the image plane has a first diffractive optics for splitting light beams into beam bundles along different orders of diffraction, imparting to the light beams a spherical phase that is different from the other orders of diffraction. A second diffractive optics is provided for the compensation of chromatic aberrations of the split beam bundles, and a collecting optics is provided for focusing split beam bundles into the image plane.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.