Polymerase variants

US10829747B2 · kind B2 · utility

Assignees

• Roche Sequencing Solutions, Inc. · US
• Roche Molecular Systems, Inc. · US

Inventors

• Aruna Ayer · Santa Clara, US
• Cleoma Arnold · San Jose, US
• Charles Wayan Schwab · San Ramon, US
• Preethi Sarvabhowman · Santa Clara, US
• Eileen Thai · San Jose, US
• Ilya Lederman · San Francisco, US
• Colin Alexander McGaw · Santa Clara, US
• Evan Amato · Menlo Park, US
• Barbara Eckert · Corona, US
• Shawn Suko · El Sobrante, US
• Mara Boenitz-Dulat · Tutzing, DE
• Bigna Woersdoerfer · Munich, DE
• David Daniel Wunderlich · Penzberg, DE

Key dates

• Filing date: Sep 20, 2017
• Grant date: Nov 10, 2020
• Expiry date: Sep 20, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2565/631
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.