Determining the arrangement of a sample object by means of angle-selective illumination

US10921573B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Lars Stoppe · Jena, DE
• Thomas Ohrt · Golmsdorf, DE
• Markus Sticker · Jena, DE

Key dates

• Filing date: Oct 20, 2017
• Grant date: Feb 16, 2021
• Expiry date: Oct 20, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/30024
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method for determining an arrangement of a sample object in an optical device comprises illuminating the sample object from at least one first illumination direction and illuminating the sample object from at least one second illumination direction. The method also comprises carrying out a correlation between data which are indicative of at least one section of an image of the sample object under illumination from the at least one first illumination direction and data which are indicative of at least one section of an image of the sample object under illumination from the at least one second illumination direction. The method also comprises determining the arrangement of the sample object in relation to a focal plane of the optical device based on the correlation.