Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
US10927144B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 14, 2008 |
| Grant date | Feb 23, 2021 |
| Priority date | — |
| Expiry date | May 25, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/065
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant are described, which methods comprise the sequential steps of: (a) passing the composition through an ion exchange membrane, where the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pI of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and the at least one contaminant, and (b) recovering the purified polypeptide from the effluent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.