Methods and compositions for sequencing

US10968536B2 · kind B2 · utility

Assignees

• JUMPCODE GENOMICS, INC. · US
• THE SCRIPPS RESEARCH INSTITUTE · US

Inventors

• Keith Brown · Niskayuna, US
• Daniel R. Salomon · San Diego, US
• Steven R. Head · Manchester, US
• Azeem Siddique · Village of La Jolla, US
• Phillip Ordoukhanian · San Diego, US

Key dates

• Filing date: Feb 25, 2016
• Grant date: Apr 6, 2021
• Expiry date: Feb 25, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1093
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.