Functional screening with optimized functional CRISPR-Cas systems
US11001829B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 24, 2017 |
| Grant date | May 11, 2021 |
| Priority date | — |
| Expiry date | Mar 24, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/20
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.