Systems and methods for multi-view nonlinear optical imaging for improved signal-to-noise ratio and resolution in point scanning multi-photon microscopy

US11041807B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Hari Shroff · Rockville, US
• Yicong Wu · North Potomac, US

Key dates

• Filing date: Aug 22, 2016
• Grant date: Jun 22, 2021
• Expiry date: Jan 8, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B2207/114
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Various embodiments of a multi-photon microscopy system that uses sequential excitation of a sample through three or more objective lenses oriented at different axes intersecting the sample are disclosed. Each objective lens is capable of focused sequential excitation of the sample that elicits fluorescence emissions from the excited sample, which is then simultaneously detected by each respective objective lens along a respective longitudinal axis every time the sample is illuminated through only a single objective lens.