Device and method for providing illumination for total-internal-reflection fluorescence microscopy using opaque mask
US11047799B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 23, 2017 |
| Grant date | Jun 29, 2021 |
| Priority date | — |
| Expiry date | Jul 7, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B27/56
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A lighting device for total-internal-reflection fluorescence microscopy includes a substrate that is transparent to light, having a refractive index higher than that of water; a light-emitting device arranged in the interior of the substrate, suitable for emitting light radiation in the direction of a surface of the substrate, the light-emitting device being arranged such that at least one portion of the radiation reaches the surface with an angle of incidence larger than or equal to a critical angle of total internal reflection for an interface between the substrate and water; and at least one opaque mask, arranged in the interior or on the surface of the substrate so as to intercept a portion of the radiation that, in the absence of the mask, would reach the surface with an angle of incidence smaller than the critical angle. A lighting device to total-internal-reflection fluorescence microscopy is provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.