Layered microfluidic array

US11118150B2 · kind B2 · utility

Assignees

• RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK · US
• Memorial Sloan Kettering Cancer Center · US

Inventors

• Sihong Wang · Scarsdale, US
• A. H. Rezwanuddin Ahmed · New York, US
• Xuejun Jiang · Cresskill, US
• Chun-wei Chi · Taipei, TW
• Chenghai Li · Beijing, CN

Key dates

• Filing date: Jan 10, 2019
• Grant date: Sep 14, 2021
• Expiry date: Jan 10, 2039

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2300/168
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A layered, microfluidic array is disclosed. The array has a first layer with a culture channels extending in a first longitudinal direction. Each culture channel includes multiple traps that entrap cell or tissue samples. The array also has a second layer with microfluidic channels extending in a second longitudinal direction that is orthogonal the first longitudinal direction. A third layer, disposed between the first layer and the second layer, has pores arranged within the third layer such that each nest is vertically stacked above, and fluidly connected with, a corresponding culture chamber in the first layer. Each nest is fluidly isolated from adjacent nests by a fluid impermeable region of the third layer such that horizontal diffusion of water within the third layer is prevented.