Functional genomics using CRISPR-Cas systems, compositions, methods, screens and applications thereof
US11149267B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 28, 2016 |
| Grant date | Oct 19, 2021 |
| Priority date | — |
| Expiry date | Apr 28, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/31
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention generally relates to libraries, kits, methods, applications and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. The present invention also relates to rules for making potent single guide RNAs (sgRNAs) for use in CRISPR-Cas systems. Provided are genomic libraries and genome wide libraries, kits, methods of knocking out in parallel every gene in the genome, methods of selecting individual cell knock outs that survive under a selective pressure, methods of identifying the genetic basis of one or more medical symptoms exhibited by a patient, and methods for designing a genome-scale sgRNA library.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.