Production of thioesters using sortase

US11162088B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Martin Schatte · Waldbronn, DE
• Mara Boenitz-Dulat · Tutzing, DE
• Frank Bergmann · Iffeldorf, DE

Key dates

• Filing date: Mar 23, 2018
• Grant date: Nov 2, 2021
• Expiry date: Mar 23, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y304/2207
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Herein is reported a method for the enzymatic production of a thioester comprising incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue), ii) a second polypeptide that has at its N-terminus a cysteine amino acid residue or is a cysteinyl compound, and iii) a third polypeptide with sortase A activity.