Patent · US Active

Transamidation reaction in deep eutectic solvents

US11174502B2 · kind B2 · utility

0Cited by

2References

10Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Martin Schatte · Waldbronn, DE
Mara Boenitz-Dulat · Tutzing, DE

Key dates

Filing date	Mar 23, 2018
Grant date	Nov 16, 2021
Priority date	—
Expiry date	Mar 23, 2038

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y304/2207
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Herein is reported a method for the enzymatic production of a polypeptide comprising the step of incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue) or LPXTA (SEQ ID NO: 41, wherein X can be any amino acid residue), ii) a second polypeptide that has i) a glycinyl, an alaninyl, or a cysteinyl compound at its N-terminus, or ii) an oligoglycine, or oligoalanine, or a cysteine amino acid residue followed by one to three glycine or alanine amino acid residues at its N-terminus, or iii) a lysine amino acid residue within its 5 N-terminal amino acid residues, and iii) a third polypeptide with sortase A activity, in a deep eutectic solvent and thereby producing a polypeptide.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.