Compositions and methods relating to a mutant Clostridium difficile toxin

US11208633B2 · kind B2 · utility

Assignee

• PFIZER INC. · US

Inventors

• Jason Arnold Lotvin · Union, US
• Annaliesa Sybil Anderson · Upper Saddle River, US
• Robert G. K. Donald · South Orange, US
• Michael James Flint · Decatur, US
• Narender Kumar Kalyan · Ridgewood, US
• Kathrin Ute Jansen · Doylestown, US
• Maninder K. Sidhu · New City, US
• Justin Keith Moran · Valley Cottage, US
• Mark Edward Ruppen · Garnerville, US
• Weiqiang Sun · Morristown, US

Key dates

• Filing date: Dec 5, 2019
• Grant date: Dec 28, 2021
• Expiry date: Dec 5, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N1/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. The mutant toxin may include a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to methods and compositions for use in culturing Clostridium difficile and in producing C. difficile toxins.