Patent · US Active

Method and microscope for determining a fluorescence intensity

US11231576B2 · kind B2 · utility

0Cited by

1References

25Claims

0Family size

Assignee

Carl Zeiss Microscopy GmbH · DE

Inventors

Christian Franke · Dresden, DE
Markus Sauer · Mils, AT
Sebastian van de Linde · Bearsden, GB

Key dates

Filing date	Nov 20, 2017
Grant date	Jan 25, 2022
Priority date	—
Expiry date	Nov 1, 2038

Classification

Technology area (CPC G)Physics
CPC primaryG02B21/361
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Method and microscope for determining a fluorescence intensity which is integrated over a partial region of an isolated intensity distribution, from a first individual image is corrected by means of a background intensity value, integrated over the same image region, from another individual image in which this image region is free from fluorescence. The axial position can be ascertained on the basis of two thus corrected fluorescence intensity values from, in each case, different partial regions of the intensity distribution.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.