Method for producing monomeric and multimeric molecules and uses thereof

US11254728B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Johannes Auer · Schwaigen, DE
• Martin Bader · Penzberg, DE
• Stefan Dengl · Munich, DE
• Stefan Lorenz · Penzberg, DE
• Stefan Seeber · Penzberg, DE

Key dates

• Filing date: Jan 30, 2020
• Grant date: Feb 22, 2022
• Expiry date: Jan 30, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Herein is reported a method for the production of a polypeptide that is biologically active as n-mer comprising a nucleic acid encoding a fusion polypeptide according to the following formula (Bn-CSo-Is-CSp-FC-CSq-It-CSr-Bm)u, wherein B denotes a polypeptide that is biologically active as n-mer and forms non-defined aggregates/multimers upon expression in the absence of a fused Fc-region, FC denotes a heavy chain Fc-region polypeptide, CS denotes a cleavage site, and I denotes an intervening amino acid sequence, wherein FC does not substantially bind to an Fc-receptor, recovering the fusion polypeptide from the cell or the cultivation medium, optionally cleaving the fusion polypeptide with a protease, and thereby producing a polypeptide that is biologically active as n-mer and forms non-defined aggregates/multimers upon expression in the absence of a fused Fc-region.