Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
US11254980B1 · kind B1 · utility
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Key dates
| Filing date | Nov 21, 2018 |
| Grant date | Feb 22, 2022 |
| Priority date | — |
| Expiry date | Dec 20, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions are provided for quantifying the number of biological input molecules of one or more target genes of interest in a PCR. The method comprises steps for amplifying multiple synthetic templates and high throughput sequencing. The method further comprises steps for achieving equal sequencing coverage between targets that do not occur in equal ratios, while mitigating the need for costly deep sequencing methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.