Methods, devices, and systems for analyte detection and analysis

US11268143B2 · kind B2 · utility

Assignee

• Ultima Genomics, Inc. · US

Inventors

• Nathan Beckett · Oakland, US
• Gilad Almogy · Givatayim, IL
• Nathan CASWELL · Sunnyvale, US
• Jacob A. Wolf · Oakland, US
• Kristopher Barbee · East Haven, US
• Denis Pristinski · Dublin, US
• Mark Pratt · Seattle, US
• Gene POLOVY · Redwood City, US
• Osip SCHWARTZ · Newark, US
• Stephanie KUBECKA · San Jose, US
• Steven M. Menchen · Fremont, US
• Joseph Anthony · Seminole, US
• Jose Martin SOSA · San Jose, US
• Phillip You Fai Lee · San Francisco, US

Key dates

• Filing date: May 5, 2021
• Grant date: Mar 8, 2022
• Expiry date: May 5, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N35/1002
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.