Glucoamylase engineered yeast and fermentation methods
US11306330B2 · kind B2 · utility
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13Claims
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Key dates
| Filing date | Feb 27, 2019 |
| Grant date | Apr 19, 2022 |
| Priority date | — |
| Expiry date | Feb 27, 2039 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32° C. or greater.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.