Methods and compositions for quantifying metabolites and proteins from single cells
US11353448B2 · kind B2 · utility
4Cited by
21References
15Claims
0Family size
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Key dates
| Filing date | Feb 16, 2016 |
| Grant date | Jun 7, 2022 |
| Priority date | — |
| Expiry date | Dec 11, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/573
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compositions for quantifying the cellular concentration of cyclic adenosine monophosphate (cAMP), cyclic guanine monophosphate (cGMP), glutathione, glucose, and glutamine in a single cell include the use of conjugates and analogs in immunofluorescent assays for on-chip quantification. These metabolite immunofluorescent assays may be incorporated with proteomic assays for simultaneous single cell analysis of metabolites and proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.