Methods and systems for determining spatial patterns of biological targets in a sample
US11359228B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 8, 2022 |
| Grant date | Jun 14, 2022 |
| Priority date | — |
| Expiry date | Feb 8, 2042 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L3/5027
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.