Method and device for optically examining a plurality of microscopic samples

US11371927B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Thomas Kalkbrenner · Jena, DE
• Joerg Siebenmorgen · Jena, DE
• Thomas Ohrt · Golmsdorf, DE

Key dates

• Filing date: Sep 25, 2018
• Grant date: Jun 28, 2022
• Expiry date: Jul 28, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/144
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for optically examining a plurality of microscopic samples. The samples are channeled one after the other by means of a flow into at least one flow channel in which the samples advance along a flow direction. The samples are illuminated, and light emitted from the samples is detected and analyzed. A device for carrying out the method in which samples are illuminated in that at least one light sheet with a light sheet plane is directed onto the at least one flow channel, wherein the light sheet is oriented so as to intersect the at least one flow channel in an intersection region, and the normal of the light sheet plane forms an angle differing from null together with the flow direction in the intersection region. The light emitted from the sample is registered by an imaging optical detection unit, and the focal plane of said optical detection unit lies in the intersection region.