Methods of growing tumor infiltrating lymphocytes in gas-permeable containers

US11401503B2 · kind B2 · utility

Assignees

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US
• Wilson Wolf Manufacturing, LLC · US

Inventors

• Steven A. Rosenberg · Potomac, US
• Mark E. Dudley · Washington, US
• David Stroncek · Rockville, US
• Marianna Sabatino · Los Angeles, US
• Jianjian Jin · Clarksburg, US
• Robert Somerville · Potomac, US
• John R. Wilson · Sauk Rapids, US

Key dates

• Filing date: Dec 6, 2018
• Grant date: Aug 2, 2022
• Expiry date: Jan 5, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2502/11
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.